Nucleofection keluaran terbaru

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Nucleofection is a specialized form of electroporation that enables the transfer of genetic material into a cell nucleus. The transfection occurs in the Neon Pipette tip.

Using nucleofection plasmids can be delivered efficiently to CD34 HSPCs in a relatively nontoxic manner. In addition nucleofection is a fast method because the results can be analyzed within 7 h. Moreover as the DNA quickly reaches the nucleus nucleofected cells were ready for analysis after only 26 h.

Load mix of harvested cells and molecules to be delivered eg DNA RNA protein into the Neon Pipette tip.

49 221 99199 400. Using nucleofection plasmids can be delivered efficiently to CD34 HSPCs in a relatively nontoxic manner. It is a highly efficient non-viral method of transfection that is ideally suited for working with all cell types although it is especially adept when using hard-to-transfect cell lines. Nucleofection after passage number 20 13 2Seed out 5 x 105 cells25 cm flask 14 Subculture 23 days before Nucleofection 15 Optimal confluency for Nucleofection.